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bbsi digested plasmid pspcas9bb 2a gfp px458  (Addgene inc)


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    Structured Review

    Addgene inc bbsi digested plasmid pspcas9bb 2a gfp px458
    Bbsi Digested Plasmid Pspcas9bb 2a Gfp Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bbsi+digested+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41910145-319-12-16
    Average 96 stars, based on 4089 article reviews
    bbsi digested plasmid pspcas9bb 2a gfp px458 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Gene Knockout:

    Article Title: FAM13A as potential therapeutic target in modulating TGF-β-induced airway tissue remodeling in COPD.
    Article Snippet: .. For gene knockout with CRISPR-Cas9, 50-AGCAGGATGAAGTTCGACAT-30 (IDT), which corresponded to a unique guide RNA targeting, an early canonical gene exon of human FAM13A, was cloned into BbsI-digested pX458 (Addgene 48138) to yield pX458-hFAM13A-4.2. ..

    CRISPR:

    Article Title: FAM13A as potential therapeutic target in modulating TGF-β-induced airway tissue remodeling in COPD.
    Article Snippet: .. For gene knockout with CRISPR-Cas9, 50-AGCAGGATGAAGTTCGACAT-30 (IDT), which corresponded to a unique guide RNA targeting, an early canonical gene exon of human FAM13A, was cloned into BbsI-digested pX458 (Addgene 48138) to yield pX458-hFAM13A-4.2. ..

    Clone Assay:

    Article Title: FAM13A as potential therapeutic target in modulating TGF-β-induced airway tissue remodeling in COPD.
    Article Snippet: .. For gene knockout with CRISPR-Cas9, 50-AGCAGGATGAAGTTCGACAT-30 (IDT), which corresponded to a unique guide RNA targeting, an early canonical gene exon of human FAM13A, was cloned into BbsI-digested pX458 (Addgene 48138) to yield pX458-hFAM13A-4.2. ..

    Article Title: Induction of ADAM10 by Radiation Therapy Drives Fibrosis, Resistance, and Epithelial-to-Mesenchyal Transition in Pancreatic Cancer
    Article Snippet: .. The oligos were annealed and cloned into BbsI-digested PX458 (Addgene Plasmid #48138). .. The ligations were transformed into NEB Stable Competent E. coli (NEB; cat. #C3040H) and plated on ampicillin-agar plates.

    Plasmid Preparation:

    Article Title: Induction of ADAM10 by Radiation Therapy Drives Fibrosis, Resistance, and Epithelial-to-Mesenchyal Transition in Pancreatic Cancer
    Article Snippet: .. The oligos were annealed and cloned into BbsI-digested PX458 (Addgene Plasmid #48138). .. The ligations were transformed into NEB Stable Competent E. coli (NEB; cat. #C3040H) and plated on ampicillin-agar plates.



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